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  • Title: [Tissue culture and plant regeneration of Ixeridium sonchifolium].
    Author: Tao J, Yang SH, Zhang MP, Tian YX.
    Journal: Zhongguo Zhong Yao Za Zhi; 2008 Feb; 33(4):368-71. PubMed ID: 18533487.
    Abstract:
    OBJECTIVE: To study the callus induction and plant regeneration of Ixeridium sonchifolium. METHOD: By using the orthogonal experiment design, the medium for callus induction and plant regeneration was optimized. RESULT: The optimal medium for callus induction was MS + 2, 4-D 1.5 mg x L(-1) +6-BA 1.5 mg x L(-1) +NAA 1.0 mg x L(-1) + IBA 1.5 mg x L(-1) + KT1.5 mg x L(-1), the optimal medium for inducing adventitious bud was MS +2, 4-D 0.2 mg x L(-1) +6-BA 0.5 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 0.5 mg x L(-1) + KT 0.5 mg x L(-1). Plantlets were rooted on 1/4MS medium supplemented with different concentrations of IBA, and high rooting and survival was achieved when the IBA concentration was 0.1 mg L(-1). CONCLUSION: An efficient system for plant regeneration of I. sonchifolium was established.
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