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  • Title: An efficient method for purification of PCR products for sequencing.
    Author: Ma H, Difazio S.
    Journal: Biotechniques; 2008 Jun; 44(7):921-3. PubMed ID: 18533902.
    Abstract:
    A high-throughput DNA sequencing method that generated high quality data was developed. A frame fashioned from a standard agarose gel combined with 0.1%-0.2% low-melting point (LMP) agarose gel was used to isolate the PCR product of interest. Collected PCR products were centrifuged without any reagents and the supernatants were directly used for a sequencing reaction. This method is simple and labor efficient, provides high quality sequences at a low cost, and bypasses problems with impure PCR products. This technique has been used for single nucleotide polymorphism (SNP) discovery in Populus angustifolia trees.
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