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  • Title: Ultra-performance liquid chromatography-tandem mass spectrometry for the determination of lacidipine in human plasma and its application in a pharmacokinetic study.
    Author: Tang J, Zhu R, Zhao R, Cheng G, Peng W.
    Journal: J Pharm Biomed Anal; 2008 Aug 05; 47(4-5):923-8. PubMed ID: 18534803.
    Abstract:
    A selective, rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for the quantification of lacidipine in human plasma. With nifedipine as an internal standard, sample pretreatment involved a simple liquid-liquid extraction with tert-butyl methyl ether of 1 ml plasma. The analysis was carried out on an Acquity UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 microm) with flow rate of 0.28 ml/min. The mobile phase was 30 mM ammonium acetate buffer-acetonitrile (18:82, v/v, pH 5.5). The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 0.025-10.000 ng/ml, with a lower limit of quantification of 0.025 ng/ml. The intra- and inter-day precision (R.S.D.) values were below 15% and accuracy (RE) was -12.7% to 11.9% at all QC levels. The method was successfully applied to a clinical pharmacokinetic study of lacidipine in healthy volunteers following oral administration.
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