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  • Title: New high-performance liquid chromatographic method for serum analysis of oxazepam: application to bioequivalence and pharmacokinetic study.
    Author: Kazemifard AG, Dabirsiaghi A, Gholami K, Javadzadeh N.
    Journal: Acta Pol Pharm; 2007; 64(4):287-93. PubMed ID: 18536153.
    Abstract:
    A rapid and sensitive high-performance liquid chromatographic method was developed and validated for determination of oxazepam in serum. Oxazepam was isolated from biological fluid using a simple liquid-liquid extraction with dichloromethane. Nordazepam was used as the internal standard. The chromatographic separation was accomplished using a 125 x 4-mm (inner diameter) stainless-steel (5 microm) Perfectsil Target ODS-3 reversed phase column with a mobile phase consisting of ammonium dihydrogen phosphate buffer (0.05 mol x L(-1), pH 5.8) and methanol (50:50, v/v), running at a flow rate of 1.5 ml x min(-1). The absorbance of the fluent was monitored at 254 nm. The developed method resulted in totally symmetrical peaks. It has been applied to assess the pharmacokinetics of oxazepam. Also the bioequivalence of two different oxazepam preparations following oral administration in healthy volunteers was assessed by this method.
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