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  • Title: Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy.
    Author: Park JY, Kwon SH, Park JW, Park SM.
    Journal: Anal Chim Acta; 2008 Jun 30; 619(1):37-42. PubMed ID: 18539171.
    Abstract:
    We report preparation of a novel platform for effective DNA hybridization and its application to the detection of single mismatched DNA. Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top of each dendrimer so that enough space would be secured for effective hybridization. This arrangement allows each probe DNA molecule to form a natural DNA double helix upon hybridization with a target DNA molecule. The single nucleotide polymorphism at either the central or end position of the 25-mer target DNA has been shown to be effectively discriminated against on this platform from each other as well as from a complementary DNA by electrochemical impedance measurements. We also report adverse effects exerted by probe ions, Fe(CN)(6)(3-/4-), on DNA hybridization reactions. The significance of the results for the use in DNA analysis is discussed.
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