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  • Title: Expression and production of human interleukin-7 in insect cells using baculovirus expression vector system (BEVS).
    Author: Mirzaei M, Jardin B, Elias CB, Prakash S.
    Journal: Appl Biochem Biotechnol; 2008 Oct; 151(1):93-103. PubMed ID: 18546077.
    Abstract:
    Interleukin-7 (IL-7) is a glycoprotein cytokine with significant clinical and biomedical potential, such as cancer therapy and HIV infections. Earlier it has been cloned and expressed in various protein expression systems; however, they are not efficient for large-scale production. To address this inadequacy, we report in this paper the production of recombinant human interleukin-7 (hIL-7) in insect cells. A recombinant bacmid containing hIL-7 was constructed, purified, and characterized. It was used to infect Trichoplusia ni (BT1-TN-5B1/High Fivetrade mark) insect cells. Result shows that T. ni cells successfully produce hIL-7 in shake flask cultures. A scale up to 2.5-L laboratory batch bioreactor showed the efficacy of this system for large-scale production. Our results offer a highly efficient, inexpensive, and convenient system for the large-scale expression and production of recombinant hIL-7.
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