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  • Title: Affinity purification of egg yolk immunoglobulins (IgY) with a stable synthetic ligand.
    Author: Dong D, Liu H, Xiao Q, Li R.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2008 Jul 01; 870(1):51-4. PubMed ID: 18550455.
    Abstract:
    Chicken IgY (egg yolk immunoglobulin) is a functional equivalent of mammalian IgG. Traditional methods for IgY purification involve multi-step procedures that result in low recovery of IgY. After a large scale screening of our 700-member synthetic ligand library synthesized by epichlorohydrin and cyanuric chloride methods, a high efficiency ligand of IgY was found. By one-step purification with this ligand, the purity of IgY could reach 92.1%, and the recovery of IgY could reach 78.2%. This synthetic ligand had a higher binding capacity of 74.8 mg IgY/ml and had no negative effects on immunoreactivity. Remarkably, this ligand was also highly stable and could resist 1M NaOH, thus having great potential for the industrial-scale production of IgY.
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