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  • Title: Sperm head morphology is related to high deoxyribonucleic acid stainability assessed by sperm chromatin structure assay.
    Author: Zini A, Phillips S, Courchesne A, Boman JM, Baazeem A, Bissonnette F, Kadoch IJ, San Gabriel M.
    Journal: Fertil Steril; 2009 Jun; 91(6):2495-500. PubMed ID: 18555233.
    Abstract:
    OBJECTIVE: To examine the relationship between sperm strict morphology and sperm chromatin integrity. DESIGN: Prospective study. SETTING: Infertility clinic. PATIENT(S): Eighty-seven consecutive semen samples from non-azoospermic men presenting for infertility evaluation and 6 samples from fertile donors. INTERVENTION(S): Assessment of standard semen parameters and sperm chromatin structure assay (SCSA) parameters (%DFI [DNA fragmentation index] and %HDS [high DNA stainability]). Evaluation of %HDS and %DFI after treatment with dithiothreitol (a thiol-reducing agent used to decondense sperm nuclei) was also undertaken. MAIN OUTCOME MEASURE(S): Relationship between sperm strict morphology defects and SCSA parameters (%DFI and %HDS). RESULT(S): We observed significant relationships between the percentage of normal sperm forms and both %HDS (r = -0.40) and sperm motility (r = 0.32). We also found significant relationships between sperm head defects and both %HDS (r = 0.40) and sperm concentration (r = -0.39). Sperm tail, midpiece, and neck defects were not significantly related to the SCSA parameters. Treatment of spermatozoa with dithiothreitol (to induce decondensation) resulted in a substantial increase in %HDS but no measurable change in %DFI. CONCLUSION(S): The observed relationship between sperm head defects and %HDS suggests that sperm head abnormalities may, in part, be due to incomplete sperm chromatin condensation.
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