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  • Title: Marked effect of RhoA-specific shRNA-producing plasmids on neurite growth in PC12 cells.
    Author: Fan YM, Pang CP, Harvey AR, Cui Q.
    Journal: Neurosci Lett; 2008 Aug 01; 440(2):170-5. PubMed ID: 18556119.
    Abstract:
    In order to promote neurite outgrowth, we constructed a plasmid producing RhoA-specific small hairpin RNA (shRNA) to block RhoA expression and tested its actions in PC12 cells. Our results show that the shRNA-mediated RNA interference (RNAi) successfully suppressed RhoA expression. As a consequence of RhoA knockdown, F-actin levels were significantly reduced and processes were markedly induced. These processes express two neurite markers, neurofilament and betaIII tubulin. This study demonstrates that plasmid-producing shRNA specific for RhoA can act as an effective tool to induce neurite outgrowth and further confirms the neurite growth-inhibitory role of RhoA. This shRNA may thus be a useful tool in promoting neurite outgrowth and could be applicable in neural repair after CNS injury.
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