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  • Title: Development of a screening method for genetically modified soybean by plasmid-based quantitative competitive polymerase chain reaction.
    Author: Shimizu E, Kato H, Nakagawa Y, Kodama T, Futo S, Minegishi Y, Watanabe T, Akiyama H, Teshima R, Furui S, Hino A, Kitta K.
    Journal: J Agric Food Chem; 2008 Jul 23; 56(14):5521-7. PubMed ID: 18558691.
    Abstract:
    A novel type of quantitative competitive polymerase chain reaction (QC-PCR) system for the detection and quantification of the Roundup Ready soybean (RRS) was developed. This system was designed based on the advantage of a fully validated real-time PCR method used for the quantification of RRS in Japan. A plasmid was constructed as a competitor plasmid for the detection and quantification of genetically modified soy, RRS. The plasmid contained the construct-specific sequence of RRS and the taxon-specific sequence of lectin1 (Le1), and both had 21 bp oligonucleotide insertion in the sequences. The plasmid DNA was used as a reference molecule instead of ground seeds, which enabled us to precisely and stably adjust the copy number of targets. The present study demonstrated that the novel plasmid-based QC-PCR method could be a simple and feasible alternative to the real-time PCR method used for the quantification of genetically modified organism contents.
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