These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Combined stimulation of nasal polyp fibroblasts with poly IC, interleukin 4, and tumor necrosis factor alpha potently induces production of thymus- and activation-regulated chemokine.
    Author: Nonaka M, Ogihara N, Fukumoto A, Sakanushi A, Pawankar R, Yagi T.
    Journal: Arch Otolaryngol Head Neck Surg; 2008 Jun; 134(6):630-5. PubMed ID: 18559731.
    Abstract:
    OBJECTIVE: To examine the effects of cytokines and poly IC on the expression of thymus- and activation-regulated chemokine (TARC), a potent chemoattractant for helper T-cell type 2 (T(H)2) cells, in nasal polyp fibroblasts. DESIGN: Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. SETTING: Academic research. PARTICIPANTS: Primary fibroblast lines were established from human nasal polyp biopsy tissue specimens (n = 5) removed at polypectomy. MAIN OUTCOME MEASURES: The expression of TARC messenger RNA (mRNA) was evaluated by real-time RT-PCR. The amount of TARC in the supernatants was measured by enzyme-linked immunosorbent assay. RESULTS: Combined stimulation with interleukin 4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) or with poly IC and IL-4 induced TARC production. Combined exposure of cells to poly IC, IL-4, and TNF-alpha resulted in substantial amounts of TARC release into the culture medium. Quantitative RT-PCR analysis revealed that simultaneous stimulation with those 3 compounds induced a tremendous increase in the amount of TARC mRNA in the nasal polyp fibroblasts. CONCLUSION: Nasal polyp fibroblasts contribute to T(H)2 cell infiltration and RNA virus-induced exacerbation of T(H)2-type airway inflammatory conditions such as allergic chronic sinusitis.
    [Abstract] [Full Text] [Related] [New Search]