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  • Title: Capillary gas chromatographic method for mandelic and phenylglyoxylic acids in urine.
    Author: Dills RL, Wu RL, Checkoway H, Kalman DA.
    Journal: Int Arch Occup Environ Health; 1991; 62(8):603-6. PubMed ID: 1856017.
    Abstract:
    In support of an occupational investigation of styrene exposure, a capillary gas chromatographic method was developed for the quantitation of the styrene metabolites mandelic and phenylglyoxylic acids. The method was based on that of Guillemin and Bauer, in which phenylglyoxylic acid was converted to mandelic acid by reduction before instrumental analysis. The earlier method had to be modified for use with capillary columns; the resulting method was sensitive, selective and reproducible. The detection limit was approximately 0.001 mg/ml urine. Approximately less than 5% relative precision was achieved in the range of 0.05-2 mg/ml urine. Mandelic acid was resolved from other components of urine and from by-products of derivatization.
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