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  • Title: Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae.
    Author: Lalitha P, Siti Suraiya MN, Lim KL, Lee SY, Nur Haslindawaty AR, Chan YY, Ismail A, Zainuddin ZF, Ravichandran M.
    Journal: J Microbiol Methods; 2008 Sep; 75(1):142-4. PubMed ID: 18579241.
    Abstract:
    A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively.
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