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  • Title: Production of a thermophilic maltooligosyl-trehalose synthase in Lactococcus lactis.
    Author: Cimini D, De Rosa M, Panariello A, Morelli V, Schiraldi C.
    Journal: J Ind Microbiol Biotechnol; 2008 Oct; 35(10):1079-83. PubMed ID: 18594890.
    Abstract:
    The thermoacidophilic archaeon Sulfolobus solfataricus MT4 encodes a maltooligosyltrehalose synthase (MTS), that catalyzes an intramolecular transglycosylation process converting the glycosidic linkages at the reducing end of dextrins from alpha-1,4 into alpha-1,1. In this research the gene encoding MTS was cloned and expressed in Lactococcus lactis NZ9000 using the so-called NICE system. Growth conditions of the recombinant strain were optimized in flask experiments in relation to enzyme production. Batch experiments in 2 L-fermenters were performed on the best identified semidefined medium and 256 U L(-1) of recombinant MTS were produced. Purified recombinant MTS shows its optimal activity at 70 degrees C and pH 5.5, prefers maltoheptaose and maltohexaose as substrates, and demonstrates minimal side hydrolytic activity.
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