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  • Title: Genetic association of acidic mammalian chitinase with atopic asthma and serum total IgE levels.
    Author: Chatterjee R, Batra J, Das S, Sharma SK, Ghosh B.
    Journal: J Allergy Clin Immunol; 2008 Jul; 122(1):202-8, 208.e1-7. PubMed ID: 18602573.
    Abstract:
    BACKGROUND: In view of the hygiene hypothesis and the involvement of acidic mammalian chitinase (CHIA) in the effector responses of IL-13 with asthma, CHIA (GeneID-27159) is a potential asthma candidate gene. OBJECTIVE: To investigate the association of CHIA polymorphisms with atopic asthma and serum total IgE levels. METHODS: Twenty-one single nucleotide polymorphisms were identified by sequencing DNA of 60 individuals. On the basis of linkage disequilibrium, 6 polymorphisms were selected and genotyped in unrelated atopic patients with asthma (N = 270) and controls (N = 292) and an independent pediatric cohort (patients, 150; controls, 101). Electrophoretic mobility shift assay and reporter gene assays were also performed. RESULTS: The rs3806448G/A promoter polymorphism showed significant association with atopic asthma (P(adult) = .00001 and P(pediatric) = .0002) and serum total IgE (P < .05). Also rs2282290G/A was associated with atopic asthma (P(adult) = .00009 and P(pediatric) = .00003), whereas the rs10494132C/T polymorphism was associated with serum total IgE in the patients (P < .05). We also showed that the promoter single nucleotide polymorphisms altered the transcriptional activity of CHIA promoter and the C to T substitution at rs10494132 abrogated the Octamer transcription factor-1 (Oct-1) binding site. CONCLUSION: Our results establish a significant association of CHIA with atopic asthma and serum total IgE levels in the Indian population.
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