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  • Title: Investigation of the role of CD1d-restricted invariant NKT cells in experimental choroidal neovascularization.
    Author: Hijioka K, Sonoda KH, Tsutsumi-Miyahara C, Fujimoto T, Oshima Y, Taniguchi M, Ishibashi T.
    Journal: Biochem Biophys Res Commun; 2008 Sep 12; 374(1):38-43. PubMed ID: 18606153.
    Abstract:
    Choroidal neovascularization (CNV) is directly related to visual loss in age-related macular degeneration and other macular disorders. We have investigated the role of CD1d-restricted invariant natural killer T (NKT) cells in laser-induced experimental CNV. Quantitative real-time PCR detected increased expression of NKT cell-related genes (Valpha14 and CXCL16) in whole eyes undergoing CNV, indicating local accumulation of NKT cells. We found a significant reduction of CNV and lower concentrations of vascular endothelial growth factor (VEGF) in ocular fluid in two different NKT cell-deficient mice, CD1d knockout (KO) and Jalpha18 KO mice. We also established in vitro co-cultures of retinal pigment epithelial cells and splenic NKT cells, and confirmed NKT cells could produce VEGF in the dish. Moreover, inoculating alpha-galactosylceramide, the ligand for NKT cells, into the vitreous cavity of C57BL/6 mice promoted CNV. We concluded that NKT cells play an important role in CNV as an inducer of VEGF.
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