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  • Title: Glycosylation of daidzein by the Eucalyptus cell cultures.
    Author: Shimoda K, Sato N, Kobayashi T, Hamada H, Hamada H.
    Journal: Phytochemistry; 2008 Sep; 69(12):2303-6. PubMed ID: 18606427.
    Abstract:
    The sequential glycosylation of a soybean isoflavone, daidzein, with cultured suspension cells of Eucalyptus perriniana and cyclodextrin glucanotransferase was studied. Daidzein was converted into two glycosylation products, daidzein 7-O-beta-D-glucopyranoside (39%) and daidzein 7-O-[6-O-(beta-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-gentiobioside, 6%), by cultured E. perriniana cells. Further glycosylation of daidzein 7-O-beta-glucoside with cyclodextrin glucanotransferase gave daidzein 7-O-[4-O-(alpha-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-maltoside, 26%), daidzein 7-O-beta-maltotrioside (15%), and daidzein 7-O-beta-maltotetraoside (7%).
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