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  • Title: [The fabrication of hepatitis B electrochemical immunosensor array].
    Author: Dai X, Liu Z.
    Journal: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2008 Apr; 25(2):450-3. PubMed ID: 18610640.
    Abstract:
    Based on integrate circuit (IC) technology, an eight-channel gold electrodes (GEs) array was developed. The immunosensor array is prepared by co-immobilizing thionine and Hepatitis B (HB) antibody on the gold electrodes through covalently binding them to GEs with a cysteamine/glutaraldehyde linkage. Hepatitis B surface antigen (HBsAg) was detected qualitatively and quantitatively by the peak current decrease percentage of the thionine. HBsAg positive/negative standard serum was well defined by the array. 8-channel synchronous detection for HBsAg was noted to be of good accuracy and reliability. The results of its clinical application were in good agreement with the results from ELISA.
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