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  • Title: Oxygen effect on lactose catabolism by a Leuconostoc mesenteroides strain: modeling of general O2-dependent stoichiometry.
    Author: Plihon F, Taillandier P, Strehaiano P.
    Journal: Biotechnol Bioeng; 1996 Jan 05; 49(1):63-9. PubMed ID: 18623554.
    Abstract:
    Lactose metabolism of a Leuconostoc mesenteroides strain was studied in batch cultures at a pH of 6.5 and 30 degrees C in 10 L of a modified MRS (De Man, Rogosa, Sharp) broth. The end products of this heterolactic bacterium were D-lactate, acetate, ethanol, and carbon dioxide. To test the effect of oxygen on their synthesis, the medium was sparged with different gases: nitrogen, air, and pure oxygen. When oxygen was available, oxygen uptake occurred, which caused a modification in acetate and ethanol production but not in lactate or carbon dioxide production; acetate plus ethanol together were produced in constant amounts, which were independent of the level of aeration. The influence of oxygen on end-product formation could be summed up by the general equation: lactose + x O(2) --> 2 D-lactate + (x + 0.1) acetate + (2 - x) ethanol + 2 CO(2). Maximal oxygen uptake (x = 2) was reached under a 120 L/h flow rate of pure oxygen. In addition, this equation provided useful information on the possible pathway of galactose catabolism by a heterofermentative microorganism.
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