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  • Title: Production and isolation of chitosan by submerged and solid-state fermentation from Lentinus edodes.
    Author: Crestini C, Kovac B, Giovannozzi-Sermanni G.
    Journal: Biotechnol Bioeng; 1996 Apr 20; 50(2):207-10. PubMed ID: 18626937.
    Abstract:
    A method for the laboratory-scale production and isolation of chitosan (polyglucosamine) by liquid and solidstate fermentation from Lentinus edodes was developed. The yields of isolated chitosan were 120 mg/L of fermentation medium under liquid fermentation conditions and 6.18 g/kg of fermentation medium under solid-state fermentation conditions. The latter method, which gives up to 50 times yields than other chitosan production methods from fungi, provides a new flexible and easily scaledup procedure for the production of low acetylation degree chitosan.
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