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  • Title: [Construction and characterization of immune combinatorial cDNA library of mouse variable immunoglobuline genes].
    Author: Pavlova MV, Hil'chuk PV, Pokholenko IaO, Nikolaiev IuS, Kordium VA.
    Journal: Tsitol Genet; 2008; 42(2):10-5. PubMed ID: 18630114.
    Abstract:
    A cDNA combinatorial antibody library of mouse variable immunoglobulin fragments has been constructed from mice immunized with rhIFN-beta1b. For this purpose, cDNAS of immunoglobulin variable heavy (V(H)) and variable light (V(L)) chains genes amplified from splenocytes were joined with linker DNA to form ScFv's (single-chain Fv-antibodies). The obtained ScFv-DNA pool was cloned into a phagemid vector and used for Esherichia coli transformation. Using the phage display technique, bacterial clones producing single-chain antibodies specific to rhIFN-beta1b were selected. The following characteristics of the combinatorial library were determined in this work: abundance, functional size, and the initial ScFv-DNA diversity in the library constructed. High specificity of interaction between phage displayed ScFv's and rhIFN-beta1b has been demonstrated.
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