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  • Title: Characterization of ISApl1, an insertion element identified from Actinobacillus pleuropneumoniae field isolate in China.
    Author: Liu J, Tan C, Li J, Chen H, Xu P, He Q, Bei W, Chen H.
    Journal: Vet Microbiol; 2008 Dec 10; 132(3-4):348-54. PubMed ID: 18632228.
    Abstract:
    An insertion sequence (IS), designated ISApl1, was identified in Actinobacillus pleuropneumoniae. It was 1072 bp in length, and contained a large open reading frame (ORF), which encoded a putative transposase whose sequence was similar to that of transposases of various IS elements of the IS30 family. Another small ORF, a putative antisense repressor of transposase, was located in the opposite direction of transposase. ISApl1 generated a 3-bp duplication of the target DNA and carried 24-bp inverted repeats sequence. ISApl1 was identified in the genome of a biofilm-formation negative A. pleuropneumoniae strain field isolate HB04 and inserted into an A/T rich region of the ORF of pgaC, which encoded the PGA [N-acetyl-D-glucosamine residues in beta(1,6) linkage] synthesizing N-glycosyltransferase. The genotype of the pgaC(-)/IS(+) was not altered after re-isolation from challenged mice, which indicated that this IS element was relatively stable in A. pleuropneumoniae during infection.
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