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  • Title: Transformation of Chinese hamster embryo cells with an avian sarcoma virus ts mutant.
    Author: Lacinová J, Hlozánek I.
    Journal: Folia Biol (Praha); 1976; 22(5):289-97. PubMed ID: 186335.
    Abstract:
    Chinese hamster embryo cells were transformed with temperature-sensitive mutant of the Schmidt-Ruppin strain of Rous sarcoma virus-subgroup A (ts NY68S Ra-A). The developed cell line elicited the morphological transformation at a permissive temperature of 35 degrees C, whereas after a shift to a non-permissive temperature of 40 degrees C the cells reverted to a normal phenotype. Tests for the presence of avian sarcoma group-specific antigen showed that the antigen was not expressed both at permissive and non-permissive temperature. However, the transient expression of gs antigen could be achieved by treatment with 5-IUdR only at the permissive temperature. There was a difference in the rate of 14C-2-deoxy-D-glucose uptake between cells cultured at the permissive and non-permissive temperature.
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