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  • Title: Archvillin anchors in the Z-line of skeletal muscle via the nebulin C-terminus.
    Author: Lee MA, Joo YM, Lee YM, Kim HS, Kim JH, Choi JK, Ahn SJ, Min BI, Kim CR.
    Journal: Biochem Biophys Res Commun; 2008 Sep 19; 374(2):320-4. PubMed ID: 18639526.
    Abstract:
    Z-Line of skeletal muscle is a complex protein network that likely plays an important role in signaling and muscle homeostasis. We used the yeast two-hybrid system to search for potential novel ligands of the Z-line portion of nebulin. We found that the C-terminal region of nebulin (residues 6457-6528) interacted with the C-terminus of archvillin (residues 1419-1687). Archvillin is a membrane skeletal protein that localizes to costameres, specialized adhesion sites in muscle. The binding sites between nebulin and archvillin were characterized using the yeast two-hybrid system, in vitro pull-down assays, and colocalization experiments in COS-7 cells. Our data suggest a model in which archvillin attaches directly to the Z-line through an interaction with the nebulin C-terminus. The interaction between nebulin and archvillin may provide a direct link between the sarcolemma and myofibrillar Z-lines.
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