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  • Title: Simultaneously subtyping of all influenza A viruses using DNA microarrays.
    Author: Han X, Lin X, Liu B, Hou Y, Huang J, Wu S, Liu J, Mei L, Jia G, Zhu Q.
    Journal: J Virol Methods; 2008 Sep; 152(1-2):117-21. PubMed ID: 18639939.
    Abstract:
    Rapid diagnosis of novel emerging subtypes of influenza viruses is vital for effective global influenza surveillance. To this end, a novel microarray based surveillance was developed for subtyping all influenza A viruses on one chip. Using reference strains of different influenza subtypes and samples from different areas, the results show that all the subtypes of the influenza A virus could be identified simultaneously on this microchip with high sensitivity. There was no cross-hybridization reaction with other viruses, indicating that the microarray is specific for influenza A viruses. Such a diagnostic microarray will undoubtedly be useful for identifying novel influenza A virus subtypes.
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