These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination of 1-kestose and nystose in vegetables and food products by high performance anion exchange chromatography coupled with pulsed amperometric detection].
    Author: Li J, Wang Z, Yang Y.
    Journal: Wei Sheng Yan Jiu; 2008 May; 37(3):359-61, 371. PubMed ID: 18646541.
    Abstract:
    OBJECTIVE: A methods for the simultaneous determination for 1-kestose (GF2) and nystose (GF3) in foods and vegetables by high performance anion exchange chromatography with integrated pulsed amperometric detection (HPAEC-PAD) was developed in this study. METHODS: With a gradient program, and NaOH and sodium acetate as mobile phase, the two fructo-oligosaccharides (FOS) were separated on a CarboPac PA1 column and then detected by a gold electrode. RESULTS: The linear range were 1.22 - 15.20 microg/g and 1.64 - 18.30 microg/g for GF2 and GF3, and the calculated method detection limit (MDL) were 0.085 microg/g and 0.089 microg/g, respectively. The relative standard deviation of GF2 (6.08 microg/g) and GF3 (7.32 microg/g) were 2.80% and 1.68% (n = 6). The contents of the two fructo- oligosaccharides in chicory root, artichoke and oral liquid were also determined. The results shows chicory root possess the highest GF2 and GF3 contents in all vegetable tissues, 6.61g/100g dw and 6.51g/100g dw, whereas the highest composition of kestose and nystose in food was found in Quantum FOS powder (raw material for additives), 34.13g/100g and 32.12g/100g respectively. The method recoveries of GF2 and GF3 were 93.97% and 97.14%. CONCLUSION: This method was sensitive, selective, and simple for FOS quantification in vegetables and food products.
    [Abstract] [Full Text] [Related] [New Search]