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  • Title: [The breeding of Phellinus baumii by protoplast fusion].
    Author: Zeng NK, Wang QY, Su MS.
    Journal: Zhong Yao Cai; 2008 Apr; 31(4):475-8. PubMed ID: 18661814.
    Abstract:
    OBJECTIVE: To breed good strains by protoplast fusion in Phellinus baumii. METHODS: 5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides (EPS) production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion. RESULTS: The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains. CONCLUSION: R002, R003 and R005 are fusants of the parent strains.
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