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  • Title: Electron spin resonance in membrane research: protein-lipid interactions.
    Author: Marsh D.
    Journal: Methods; 2008 Oct; 46(2):83-96. PubMed ID: 18672066.
    Abstract:
    Different electron spin resonance (ESR) methods are described that allow determination of the stoichiometry and selectivity of interaction of spin-labelled lipids with integral transmembrane peptides or proteins, and also with peripheral surface-binding membrane proteins or peptides. In addition, ESR methods for determining the exchange rates of spin-labelled lipids at the protein-lipid interface are described, as well as methods to detect penetration of surface-binding peptides into the hydrophobic membrane core. Instrumental requirements are considered, and also sample handling, spin-labelling techniques and the synthesis of spin-labelled lipids.
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