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  • Title: Imaging mass spectrometry using peptide isoelectric focusing.
    Author: Vaezzadeh AR, Simicevic J, Chauvet A, François P, Zimmermann-Ivol CG, Lescuyer P, Deshusses JP, Hochstrasser DF.
    Journal: Rapid Commun Mass Spectrom; 2008 Sep; 22(17):2667-76. PubMed ID: 18677718.
    Abstract:
    Imaging Mass Spectrometry (IMS) has emerged as a powerful technique in the field of proteomics. The use of Immobilized pH Gradient-IsoElectric Focusing (IPG-IEF) is also a new trend, as the first dimension of separation, in shotgun proteomics. We report a combination of these two outstanding technologies. This approach is based on the separation of shotgun-produced peptides by IPG-IEF. The peptides are then transferred by capillarity to a capture membrane, which is then scanned by the mass spectrometer to generate MS images. This high-throughput methodology allows a preview of the sample to be obtained in a single day. We report the application of this new pipeline for differential comparison of the membrane proteome of two different strains of Staphylococcus aureus bacteria in a proof-of-principle experiment.
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