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  • Title: Targeted metabolite analysis and antioxidant potential of Rumex induratus.
    Author: Guerra L, Pereira C, Andrade PB, Rodrigues MA, Ferreres F, De Pinho PG, Seabra RM, Valentão P.
    Journal: J Agric Food Chem; 2008 Sep 10; 56(17):8184-94. PubMed ID: 18681441.
    Abstract:
    Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic compounds and organic acids, and the study of its antioxidant activity against the DPPH(*) radical, a reactive oxygen species, hypochlorous acid, and a reactive nitrogen species, nitric oxide ((*)NO), were performed. The samples were collected in several locations, spontaneously occurring or from greenhouse culture, at different stages of development and seasons. The phenolic composition was achieved by high-performance liquid chromatography (HPLC)-diode array detection, and four hydroxycinnamic acid derivatives and 10 flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established by HPLC-UV, revealing five compounds. The total amount of phenolic compounds and organic acids were affected by growing conditions and developmental phase. The aqueous extract exhibited a dose-related activity against all tested reactive species.
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