These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Role of hemoglobin in improving biodegradation of aromatic contaminants under hypoxic conditions.
    Author: Stark BC, Urgun-Demirtas M, Pagilla KR.
    Journal: J Mol Microbiol Biotechnol; 2008; 15(2-3):181-9. PubMed ID: 18685270.
    Abstract:
    Genetic engineering of bacteria using the Vitreoscilla (bacterial) hemoglobin gene has been used to enhance bioremediation of several compounds which are models for, or are themselves, toxic chemicals which may contaminate soil and water. Initial experiments, done mostly in shake flasks, with Escherichia coli, Burkholderia sp. DNT and Pseudomonas aeruginosa demonstrated that expression of Vitreoscilla hemoglobin in heterologous hosts can enhance biodegradation of several aromatic compounds as well as an organophosphorus compound. These studies concentrated for the most part on enhancement of endogenous catabolic capabilities of the hosts; the presence of vgb/VHb enhanced both growth and biodegradation. The initial studies were followed by experiments in systems which more closely approximated conditions that would exist in field applications. These included soil columns, continuous flow reactors and membrane bioreactors. The latter work also enabled calculation of the effects of the presence of vgb/VHb on kinetic parameters such as growth rate, substrate and oxygen utilization rate, and degradation rate of pollutants, etc. Although not always the case, for the most part, and particularly in bioreactors, the advantages due to vgb/VHb were greater under conditions of limited aeration or hypoxic conditions.
    [Abstract] [Full Text] [Related] [New Search]