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  • Title: Photopolymerization as an innovative detection technique for low-density microarrays.
    Author: Kuck LR, Taylor AW.
    Journal: Biotechniques; 2008 Aug; 45(2):179-82, 184-6. PubMed ID: 18687067.
    Abstract:
    One limitation that accounts in part for the scarcity of commercially available diagnostic microarrays is the expense associated with fluorescence detection. Here we present a colorimetric method based on photopolymerization as an "on-chip" signal amplification technique. Proof of principle experiments are detailed and followed by the use of a simple influenza microarray to demonstrate the technique for the first time with clinical samples. The advantages of this new technique include rapid (<5 min) signal amplification ( approximately 105) in ambient conditions for both DNA and protein microarrays, low reagent cost (<$1 per assay), visual or inexpensive detection, and signal preservation for at least two years under ambient conditions.
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