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  • Title: [Preparation and identification of antibody against Cecropin-XJ].
    Author: Zheng ST, Liu ZY, Wang H, Dou J, Zhang FC.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2008 Aug; 24(8):791-4. PubMed ID: 18687220.
    Abstract:
    AIM: To prepare the mouse antibody against Cecropin-XJ, identify its specificity and use it in cellular localization of Cecropin-XJ in vitro. METHODS: The cDNA sequence of Cecropin-XJ with signal peptide sequence was subcloned into the eukaryotic expression vector pcDNA3. The recombinant plasmid was named pcDNA3-Cecropin-XJ, which was used as an antigen to immune the Kunming white mouse. Meanwhile, the cDNA sequence was subcloned into the fusion expression vector and the fusion protein was expressed as a test antigen. RESULTS: Indirect ELISA showed that the fifth immunity's titer was highest. The immune gold-conjugated experiment showed that the prepared antibody was clearly and efficiently localized in prokaryotic cells where the Cecropin-XJ worked. CONCLUSION: The antibody against Cecropin-XJ has high immune reactivity and specificity, which is beneficial to further study on Cecropin-XJ function and antibody preparation of small peptides.
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