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  • Title: [Freeze-drying of oxygen-carrying substances and storage stability of the freeze-dried solids].
    Author: Wang Z, Li F, Zhang H, Yu R, Yang C.
    Journal: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2008 Jun; 25(3):678-81, 693. PubMed ID: 18693455.
    Abstract:
    Oxygen-carrying substances based on poly-placental hemoglobin were put into freeze-drying. Sucrose was chosen to inhibit the methmoglobin(MetHb) formation. MetHb content, ultravioletes(UV) spectrum, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), High performance liquid chromatography (HPLC) were monitored during freeze-drying. As a result, when the mass ratio of sucrose to protein was above 0.5, MetHb formation was under control;the characteristic absorption of UV spectrum, SDS-PAGE and HPLC showed no visible change. Freeze-dried solids were kept under room temperature and refrigerator for 3 months. As a result, MetHb formation depended upon storage temperature and the mass ratio of sucrose to protein. For group C in which the mass ratio of sucrose to protein was 1.0; there is no marked change in MetHb content, UV spectrum, SDS-PAGE and HPLC after 3 months.
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