These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: [Biocatalytic properties of thermolysin immobilized on polyvinyl alcohol cryogel].
    Author: Beliaeva AV, Smirnova IuA, Lysogorskaia EN, Oksenoĭt ES, Timofeeva AV, Lozinskiĭ VI, Filippova IIu.
    Journal: Bioorg Khim; 2008; 34(4):487-94. PubMed ID: 18695721.
    Abstract:
    Preparations with different contents of thermolysin were obtained by the immobilization of the enzyme on granulated polyvinyl alcohol cryogel. Their activity and stability in an aqueous medium and in mixtures of polar organic solvents of different composition were investigated. The catalytic properties of the preparations in reactions of peptide bond formation were studied, and the optimal amount of the biocatalyst, the concentrations of initial reagents, and the ratios of organic solvents and water necessary for effective enzymatic peptide synthesis catalyzed by immobilized thermolysin were determined. A series of peptides of the general formula Z-Ala-Ala-Xaa-pNA, where Xaa = Leu, Ile, Phe, Val, or Ala, were synthesized, and the immobilized enzyme was shown to retain substrate specificity in an organic medium.
    [Abstract] [Full Text] [Related] [New Search]