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  • Title: Ossicular erosion by cholesteatoma: investigation by scanning electron microscopy utilizing a new preparation technique.
    Author: Wong J, Stoney P, Hawke M.
    Journal: J Otolaryngol; 1991 Jun; 20(3):216-21. PubMed ID: 1870172.
    Abstract:
    A new technique for removing the soft tissues from middle ear ossicles to prepare them for scanning electron microscopy is described. The technique involved use of a solution of hypochlorite. The bony surface of normal ossicles was studied as a control group after preparation by the technique and no morphological distortion was observed. Ossicles eroded by cholesteatoma were then studied, and we propose that the erosion occurs in three stages; pumicing, pitting and cavitating. The mucoperiosteum of normal ossicles and otosclerotic foci were also studied.
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