These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effect of TGF-beta1 on expression of integrin beta1 following corneal alkali burns in rabbits].
    Author: Wang F, Sun Y, Lu Y, Ma W.
    Journal: Yan Ke Xue Bao; 2008 Mar; 24(1):13-7. PubMed ID: 18709950.
    Abstract:
    PURPOSE: To observe the effect of TGF-beta1 applied topically to the alkali-injured rabbit eye on corneal epithelial wound healing and expression of integrin beta1 and its therapeutic action on corneal alkali burns. METHODS: Alkali burn was produced in 60 corneas from 30 rabbits. Two groups were randomly divided. One group was treated with TGF-beta1 solution (200 ng/ml) topically 3 times one day within the first 7 days, the other group was treated with phosphate-buffered saline (PBS) solution. The injured eyes were photographed after the fluorescence staining with a digital camera and the pictures were analyzed with computer-aided picture analysis system to calculate the rate of corneal epithelial healing. The expression of integrin beta1 was investigated in the point 6 h, 1 d, 3 d, 7 d, 14 d after the injury by means of immunohistochemical analysis. RESULTS: On the 4th, 10th, 11th, 12th and 14th days after the burning,the rate of corneal epithelial healing of TGF-beta1 groups was markedly higher than that of the PBS group (P < 0.05). The expression of integrin beta1 in the cornea epithelial cells gradually increased during the wound healing. On the 7th and 14th days after the burning, the expression of integrin beta1 in the cornea epithelial cells of TGF-beta1 group was remarkably higher than that of the PBS group (P < 0.05). CONCLUSIONS: TGF-beta1 could up-regulate integrin beta1 in vivo corneal alkali burn model, which could stimulate the cornea epithelial cells to migrate and adhere to the cornea stroma, that can reduce the cases of the epithelial cells'detachment from the cornea stroma and sustain the corneal reepithelization.
    [Abstract] [Full Text] [Related] [New Search]