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  • Title: [Isolation and physiological characteristics of lytic bacteriophages of Vibrio].
    Author: Sun J, Ye D, Kochel A, Jost G.
    Journal: Wei Sheng Wu Xue Bao; 2008 Jun; 48(6):780-4. PubMed ID: 18720843.
    Abstract:
    OBJECTIVE: Vibrio is a widely distributed pathogen in aquatic environment. Our study aimed at searching for possible biological control of pathogenic vibrio. METHODS: We collected natural samples from coast and lakes in spring of 2006 and autumn of 2005; and isolated lytic phages by double-layer plate method. We identified the hosts with 16S rDNA sequencing and observed their morphology with phages under electron microscopy. We also tested the physiological characteristics of phages. RESULTS: We isolated 96 bacteria and 2 phages (Vibio/XM/P1, Vibio/XM/P2). Their hosts belonged to Vibrio alginolyticus and Vibrio anguillarum. Both phages were hexagonal-headed and one with a tail. Physiological tests show that their optimum grow condition were pH7, 25 degrees C and pH8, 30 degrees C. Both phages were sensitive to high temperature and UV light. Vibrio/XM/P2 was sensitive to aether and chloroform whereas Vibrio/XM/P1 not.
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