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  • Title: Lacalization of the SV40 T antigen in hamster cells transformed by PARA(3ct)-adenovirus 7.
    Author: Dottorini S, Tassi C.
    Journal: Intervirology; ; 6(6):343-9. PubMed ID: 187551.
    Abstract:
    The localization of the SV40 T antigen in hamster cells transformed by the PARA(3ct)-adenovirus 7 variant and, for comparison, by SV40 was examined. The H50 cell strain (transformed by SV40) and the P7-/BL/SV/OD strain (transformed by PARA(3ct)-adenovirus 7) showed a preferential localization of the SV40 T antigen in the nucleus both by immunofluorescence and by complement fixation. On the other hand, two different PARA(3ct)-adenovirus 7-transformed cell lines (P7-/Ar/d/15A and P7/Ar/m/14A) showed an almost equivalent concentration of the SV40 T antigen in the nucleus and in the cytoplasm both by immunofluorescence and complement fixation. The H50, P7-/BL/SV/3D, P7/Ar/m/14A cells were oncogenic for hamsters; the P7/Ar/d/15A line was not.
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