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  • Title: Genetic manipulation of Leptospira biflexa.
    Author: Louvel H, Picardeau M.
    Journal: Curr Protoc Microbiol; 2007 Jun; Chapter 12():Unit 12E.4. PubMed ID: 18770609.
    Abstract:
    The genus Leptospira belongs to the order Spirochaetales and is composed of both saprophytic and pathogenic members, such as Leptospira biflexa and L. interrogans, respectively. A major factor contributing to our ignorance of spirochetal biology has been the lack of methods available for genetic analysis of these organisms. In recent years, an E. coli-L. biflexa shuttle vector has been constructed and a system for targeted mutagenesis and random transposon mutagenesis of the saprophyte L. biflexa has been developed. These studies enable the use of L. biflexa as a model bacterium among spirochetes.
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