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  • Title: Northern analysis of viral plus- and minus-strand RNAs.
    Author: Palani PV, Lin NS.
    Journal: Curr Protoc Microbiol; 2007 Feb; Chapter 16():Unit 16E.3. PubMed ID: 18770617.
    Abstract:
    Replication is a fundamental activity of viruses. Replication of positive-sense RNA viruses involves the synthesis of complementary minus-strand intermediates from the parental RNA template followed by synthesis of nascent plus strands. Negative-sense RNA genome and double-stranded RNA are copied into positive-sense mRNA before translation. To detect and estimate the abundance of plus- and minus-strand viral transcripts in the infected samples, northern analysis is the most commonly used method.
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