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  • Title: Analysis of DNA denaturation.
    Author: Darzynkiewicz Z, Juan G.
    Journal: Curr Protoc Cytom; 2001 May; Chapter 7():Unit 7.8. PubMed ID: 18770735.
    Abstract:
    This unit provides a detailed method for evaluating in situ DNA denaturation by flow cytometry. The principal technique is based upon the metachromatic properties of acridine orange. This technique has a number of advantages over traditional biochemical methods, but requires very precise methodology; the result is excellent clarity and differentiation between single-stranded and double-stranded DNA. This unit provides full details on the correct use of acridine orange together with an excellent discussion of the pitfalls and problems.
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