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  • Title: [Construction of 2-dimensional tumor microvascular architecture phenotype in non-small cell lung cancer].
    Author: Liu JK, Wang XY, Xiong Z, Zhou H, Zhou JH, Fu CY, Li B.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2008 Aug; 33(8):712-7. PubMed ID: 18772511.
    Abstract:
    OBJECTIVE: To construct a technological platform of 2-dimensional tumor microvascular architecture phenotype (2D-TAMP) expression. METHODS: Thirty samples of non-small cell lung cancer (NSCLC) were collected after surgery. The corresponding sections of tumor tissue specimens to the slice of CT perfusion imaging were selected. Immunohistochemical staining,Gomori methenamine silver stain, and electron microscope observation were performed to build a technological platform of 2D-TMAP expression by detecting the morphology and the integrity of basement membrane of microvasculature, microvascular density, various microvascular subtype, the degree of the maturity and lumenization of microvasculature, and the characteristics of immunogenetics of microvasculature. RESULTS: The technological platform of 2D-TMAP expression was constructed successfully. There was heterogeneity in 2D-TMAP expression of non-small cell lung cancer. The microvascular of NSCLC had certain characteristics. CONCLUSION: 2D-TMAP is a key technology that can be used to observe the overall state of micro-environment in tumor growth.
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