These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Preparation of F0F1 -ATPase nanoarray by dip-pen nanolithography and its application as biosensors.
    Author: Li Z, Liu X, Zhang Z.
    Journal: IEEE Trans Nanobioscience; 2008 Sep; 7(3):194-9. PubMed ID: 18779099.
    Abstract:
    Dip-pen nanolithography (DPN) is a widely used technique to create nanoscopic patterns of many different materials. F(0)F(1) -ATPase is a nanoscale rotary molecular motor, and could be used as a biosensor or an ideal motor device in a micro-/nanosystem. In this paper, the DPN technique was used to create nanoarrays of F(0)F(1) -ATPase within chromatophore on a gold surface. The feature size of our F(0)F(1) -ATPase patterns was an average of 130 nm, and mathematically, there were no more than ten F(0)F(1) -ATPases in each dot. The biological activity of patterned F(0)F(1) -ATPase was demonstrated by its adenosine triphosphate synthesis, which was indicated by the fluorescence change of labeled F1300. The patterned F(0)F(1) -ATPase nanoarrays were further constructed as biosensors to detect H9 influenza A virus. The results showed that the biosensor arrays had a remarkable S/N ratio and excellent specificity. This type of biosensor arrays can be further used in high-throughput, high-sensitive detection in future. Meanwhile, the precise patterning of F(0)F(1) -ATPase with desired size, position, and biological activity would accelerate its application in many fields.
    [Abstract] [Full Text] [Related] [New Search]