Title: Comparison of evaporation techniques for the preparation of salivary cortisol for analysis by liquid chromatography-electrospray tandem mass spectrometry. Author: Nelson EA, Palombo EA, Knowles SR. Journal: Clin Biochem; 2008 Nov; 41(16-17):1413-6. PubMed ID: 18789315. Abstract: OBJECTIVE: Compare evaporation techniques in the work-up procedure of saliva samples for cortisol analysis by a previously validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. DESIGN AND METHODS: Samples were evaporated using freeze drying, centrifugal concentration, and nitrogen flow and analyzed by LC-MS/MS. RESULTS: Cortisol concentrations detected using each technique did not significantly differ. Factors other than the recovery of cortisol were evaluated. CONCLUSIONS: Evaporation using freeze drying/centrifugal concentration is an automated process, reducing overall processing time for analysis of numerous samples.[Abstract] [Full Text] [Related] [New Search]