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  • Title: Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode.
    Author: David F, Tienpont B, Sandra P.
    Journal: J Sep Sci; 2008 Oct; 31(19):3395-403. PubMed ID: 18792008.
    Abstract:
    The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1-D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC x GC) and by GC-MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC x GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC-EI-MS and GC-PCI-MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta, Chryseobacterium gleum and Stenotrophomonas maltophilia.
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