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  • Title: Glycation-induced crosslinking of calf lens crystallins.
    Author: van Boekel MA, Hoenders HJ.
    Journal: Exp Eye Res; 1991 Jul; 53(1):89-94. PubMed ID: 1879506.
    Abstract:
    To investigate the possible role of glycation in the onset of diabetic cataract we used calf lens crystallins as a model. After incubation with reducing sugars, the proteins were investigated by high-pressure gel permeation chromatography, SDS-PAGE and analytical ultracentrifugation. Glucose-6-phosphate incubation resulted in an increase in mean molecular weight of all crystallin fractions and the occurrence of high-molecular weight material, partly formed by disulphide bonds. The glycated crystallins showed a decrease of tryptophan fluorescence and an increase of a specific non-tryptophan fluorescence. This fluorescence was, however, not exclusively associated with the high molecular weight protein, but was present in all protein fractions.
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