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  • Title: [Rearrangements of yeast chromosomes revealed by pulsed field gel electrophoresis].
    Author: Iarovoĭ BF, Mezhevaia Ev, Stepanova VL, Zakharov IA.
    Journal: Genetika; 1991 Apr; 27(4):636-43. PubMed ID: 1879679.
    Abstract:
    Electrophoretic karyotypes of yeast Saccharomyces cerevisiae integrant strains containing the pYF91 plasmid integrated into the chromosomes I, III, VI, IX, XI were studied. A possibility was demonstrated of visual identification of the chimaeric chromosomes via the molecular weight increase by 13200 bp (the plasmid size) determined by pulsed field gel electrophoresis. Several gamma-rays induced rearrangements of the yeast chimaeric chromosome I causing instability in hybrids were also studied. The deletions induced in the I chromosome were analysed and their size estimated. The technique of pulsed field gel electrophoresis is recommended for determination of insertions and deletions in the chromosomes of yeast Saccharomyces cerevisiae.
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