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  • Title: A novel reagentless amperometric immunosensor based on gold nanoparticles/TMB/Nafion-modified electrode.
    Author: Wu Y, Zheng J, Li Z, Zhao Y, Zhang Y.
    Journal: Biosens Bioelectron; 2009 Jan 01; 24(5):1389-93. PubMed ID: 18799302.
    Abstract:
    A novel reagentless immunosensor was fabricated by immobilization of redox mediator 3,3',5,5'-tetramethylbenzidine (TMB) on the Nafion (Nf) film modified glassy carbon electrode. Gold nanoparticles were assembled onto the TMB/Nafion film modified electrode to provide active sites for the immobilization of antibody molecules. The antibody (anti-MIgG), in the present study, was fixed on the electrode for the rapid detection of antigen molecules (MIgG as a model analyte). The results showed that the immunosensor based on the immobilized TMB redox mediator exhibited good electrochemical response. A good linear relationship between peak current and the concentration of the MIgG was obtained in the concentration range from 4 to 120ng/mL. The detection limit was estimated to be 1ng/ml. Under the optimized conditions, the immunosensor exhibits good sensitivity, reproducibility and stability.
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