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  • Title: Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step.
    Author: Gupta M, Caniard A, Touceda-Varela A, Campopiano DJ, Mareque-Rivas JC.
    Journal: Bioconjug Chem; 2008 Oct; 19(10):1964-7. PubMed ID: 18800819.
    Abstract:
    We demonstrate that QDs coated with nitrilotriacetic acid (NTA) bound to Ni (2+) can be used to reversibly and selectively bind, purify, and fluorescently label His 6-tagged (N-terminal) glutathione S-transferase (GST) in one step with retention of enzymatic activity. We find binding to be less effective in the absence of the His 6-tag or Ni (2+) ions.
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